Translational_Unit

Part:BBa_K3584002:Design

Designed by: Kong Yangyang   Group: iGEM20_Shanghai_United   (2020-10-17)


T7 pro-tag-Lac ope-PPO-T7 ter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 169
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1428
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 380
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To meet the type IIS standard, we made a synonymous mutation of the Leu15Leu in the PPO coding sequence to remove the SapI restriction site.


Source

Polyphenol oxidases (EC1.10.3.1)

References